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种属 | 人 |
别称 | UMUC-3; UM-UC3; UMUC3; UC-3; University of Michigan-Urothelial Carcinoma-3 |
组织来源 | 膀胱 |
疾病 | 移行细胞癌 |
传代比例/细胞消化 | 1:2传代,消化2-3分钟 |
完全培养基配置 | MEM培养基;10%胎牛血清;1%双抗 |
简介 | Derived from a urinary bladder transitional cell carcinoma from a human male. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously. Hypertriploid. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker chromosomes in each cell. They included der(1)t (1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) and others. The X and N3 had single copy per cell, and others were generally two or three copies per cell. |
形态 | 上皮细胞样 |
生长特征 | 贴壁生长 |
倍增时间 | 每周 2 至 3 次 |
致瘤性 | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
STR | Amelogenin: X CSF1PO: 10,11 D13S317: 8 D16S539: 8,9 D5S818: 12 D7S820: 8,9 THO1: 6,9 TPOX: 10 vWA: 17 |
培养条件 | 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。 |
冻存条件 | 冻存液:90%FBS,DMSO 10%, 或使用非程序冻存液:官网货号SH-H040 |
保藏机构 | ATCC; CRL-1749 |
产品使用 | 仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。 |