T/G HA VSMC; T/G HA-vSMC; T/GHA-VSMC; TGHAVSMC; T/G Human Aortic Vascular Smooth Muscle Cells
组织来源
主动脉;平滑肌
传代比例/细胞消化
1:2传代,消化2-3分钟
完全培养基配置
The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.05 mg/ml ascorbic acid; 0.01 mg/ml insulin; 0.01 mg/ml transferrin; 10 ng/ml sodium selenite; 0.03 mg/ml Endothelial Cell Growth Supplement (ECGS); fetal bovine serum to a final concentration of 10%, HEPES to a final concentration of 10 mM, TES to a final concentration of 10 mM.